PhD positions in electron cryomicroscopy available at the Laboratory of Structural Cell Biology
Two PhD positions will be available in my newly established lab, located at the Centre of New Technologies at the University of Warsaw, Poland. The successful candidate will investigate the organisation and architecture of archaeal cells at the molecular level. We utilise a multiscale approach, combining imaging across scales (X-ray, single particle cryoEM and fluorescence light microscopy bridged by electron cryotomography) with biochemistry. More details are available at https://cent.uw.edu.pl/en/laboratories/laboratory-of-structural-cell-biology-starting-from-may-2019
The Centre of New Technologies is equipped with state-of-the-art. instrumentation including: a dedicated Akta Pure system for large scale protein purification, UPLC-coupled Orbitrap for native mass spectrometry, computing cluster consisting of 646 CPUs and 366 GPUs, a Vitrobot plunge freezer, a 200 kV Glacios cryo-TEM fitted with a phase plate and a Falcon 3EC direct electron detector. Additionaly, we will have access to a Titan Krios G3i cryo-TEM equipped with a phase plate, BioQuantum energy filter and both K3 and Falcon3EC direct electron detectors located at the National Centre for Electron Cryomicroscopy in Kraków, Poland.
There are two FIB-SEM instruments (FEI Helios, Zeiss CrossBeam) at the campus that will be upgraded with a Leica cryostage to enable sample thinning prior to cryotomography imaging by micromachining of biological specimens in cryo conditions. The working language of the institute is English.
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